Magnetic field dependence of spatial frequency encoding NMR as probed on an oligosaccharide.

Abstract

The magnetic field dependence of spatial frequency encoding NMR techniques is addressed through a detailed analysis of (1)H NMR spectra acquired under spatial frequency encoding on an oligomeric saccharide sample. In particular, the influence of the strength of the static magnetic field on spectral and spatial resolutions that are key features of this method is investigated. For this purpose, we report the acquisition of correlation experiments implementing broadband homodecoupling or J-edited spin evolutions, and we discuss the resolution enhancements that are provided by these techniques at two different magnetic fields. We show that performing these experiments at higher field improves the performance of high resolution NMR techniques based on a spatial frequency encoding. The significant resolution enhancements observed on the correlation spectra acquired at very high field make them valuable analytical tools that are suitable for the assignment of (1)H chemical shifts and scalar couplings in molecules with highly crowded spectrum such as carbohydrates.