Magnetic-driven dynamic culture promotes osteogenesis of mesenchymal stem cell

Mengyang Hao,Minghao Xiong,Wen-Song Tan,Yangyang Liu,Haibo Cai

doi:10.1186/s40643-021-00368-4

Mengyang Hao, Minghao Xiong + Show 3 more

Open Access

https://doi.org/10.1186/s40643-021-00368-4

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Abstract

Effective nutrient transport and appropriate mechanical stimulation play important roles in production of tissue-engineered bone grafts. In this study, an experimental set-up for magnetic-driven dynamic culture of cells was designed to mimic the microenvironment of the bone tissue. Here, its ability to contribute to osteogenic differentiation was investigated by inoculating human umbilical cord mesenchymal stem cells (HUMSCs) on magnetic scaffolds. The cytocompatibility of the developed magnetic scaffolds was verified for HUMSCs. Magnetic scaffolds seeded with HUMSCs were exposed to magnetic fields. The results showed that magnetic fields did not affect cell activity and promoted HUMSCs osteogenic differentiation. The magnetic scaffolds were magnetically driven for dynamic culture in the experimental set-up to evaluate the influence of HUMSCs osteoblast differentiation. The results indicated that magnetic-driven dynamic culture increased cell alkaline phosphatase (ALP) activity (p < 0.05) and calcium release (p < 0.05) compared with static culture. The effect was demonstrated in the expression of bone-associated genes. Overall, this study showed that magnetic-driven dynamic culture is a promising tool for regenerative bone engineering.

Highlights

In the current rapid development of society, bone diseases caused by inflammation, tumors and accidents have become one of the major healthcare challenges worldwide (Li et al 2018; Soundarya et al 2018)
Fourier‐transformed infrared spectroscopy (FTIR) was carried out to observe the chemical status of the scaffolds to determine the composition of scaffolds
The FTIR spectra of magnetic scaffolds had the characteristic peaks of chitosan, Fe3O4, and laponite

Summary

Introduction

In the current rapid development of society, bone diseases caused by inflammation, tumors and accidents have become one of the major healthcare challenges worldwide (Li et al 2018; Soundarya et al 2018). Using human mesenchymal stem cells (MSCs) to repair damaged bone tissue is considered to be a reliable method (Toosi et al 2018; Zhang et al 2019). Current tissue engineering bioreactors achieve dynamic culture by Recently, magnetic fields have been incorporated in tissue engineering to promote the osteogenesis of MSCs by increasing the matrix vesicle secretion and mineralization (Chang et al 2020). Some studies have shown that magnetic scaffolds driven by magnetic fields can realize dynamic culture (Filippi et al 2019). A magnetic-driven dynamic culture method using magnetic scaffolds was proposed, providing a novel way for dynamic culture without the damage to cells

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