Magnetic dispersive solid-phase extraction of clarithromycin and azithromycin by Fe3O4@ionic salt g-C3N4 adsorbent and HPLC-UV

Abstract

In this study, Fe3O4@ionic salt g-C3N4 was synthesized as a new adsorbent for magnetic dispersive solid phase extraction (MDSPE) of two antibiotics (clarithromycin and azithromycin) in biological samples followed by HPLC-UV. The new nanocomposite was characterized by Fourier-transform infrared (FT-IR), field emission-scanning electronic microscope (FE-SEM), transmission electron microscope (TEM), nitrogen adsorption–desorption analysis, X-ray powder diffraction (XRD), and vibrating sample magnetometer (VSM). Box-Behnken design (BBD) and response surface methodology (RSM) were used for the optimization and design of experiments. The method had a suitable linear range (1–300 µg/L), a high coefficient of determination (R2; 0.9988 and 0.9993), and a low detection limit (0.33 and 0.42 µg/L) for clarithromycin and azithromycin, respectively. The applicability of the method was evaluated by estimating the recovery of analytes from urine and blood serum samples. Recovery of more than 90.0 % and RSDs of less than 5.2 % show the acceptable accuracy and precision of the proposed method.