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Abstract
Pectinase was immobilized on magnetic regenerated cellulose microspheres using cationic polyacrylamide (CPAM) or polyethyleneimine (PEI) via electrostatic adsorption. PEI-immobilized enzyme (PEI-EMCB) had higher activity (2711 U/g) than CPAM-immobilized enzyme (CPAM-EMCB). The time course of PEI-immobilized enzyme catalytic reaction was similar to the free form. PEI-EMCB reached its maximal activity at pH 4.8, while the optimal pH for CPAM-EMCB was the same with the free form (4.4). CPAM3-EMCB was most tolerant to pH variation, which can be possibly ascribed to the high molecular weight of CPAM3. After immobilization, the optimum temperature for the enzyme declined from 45 °C to different degrees. PEI-EMCB exhibited good storage stability with 67% of the initial activity maintained after 7 days and with moderate reusability. The magnetic properties of the regenerated cellulose beads provided convenience for the immobilized enzyme to be used and recycled. The results indicate a potential route for utilization of cellulose as enzyme support via a simple method.
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