Abstract
BackgroundPatients with periodontitis seek periodontal-orthodontic treatment to address certain functional and aesthetic problems. However, little is known of the effect of periodontitis on orthodontic treatment. Thus, we compared the differences in peptide mass fingerprints of orthodontic patients with and without periodontitis by MALDI-TOF MS using a magnetic bead-based peptidome analysis of saliva samples. In this way, we aimed to identify and explore a panel of differentially-expressed specific peptides.ResultsSaliva samples from 24 patients (eight orthodontic patients without periodontitis, eight with periodontitis and another eight with periodontitis but no orthodontic treatment) were analyzed, and peptide mass fingerprints were created by scanning MS signals using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with magnetic beads. Nine mass peaks showed significant differences. Orthodontic patients in the group without periodontal disease showed higher mass peaks for seven peptides of the nine, whereas the mass peaks for the other two peptides were higher in the periodontal-orthodontic patients. Besides, these differentially-expressed peptides were sequenced.ConclusionsThe elucidated candidate biomarkers indicated interactions between periodontal condition and orthodontic treatment and their contributions to the changes of saliva protein profiles. Our results provide novel insight into the altered salivary protein profile during periodontal-orthodontic treatment, and may lead to the development of a therapeutic monitoring strategy for periodontics and orthodontics.
Highlights
Patients with periodontitis seek periodontal-orthodontic treatment to address certain functional and aesthetic problems
Saliva peptidome fingerprint peaks were characterized in each patient by showing the maximum intensity within a particular m/z range
Some authors argue that orthodontic treatment is unsuitable in chronic periodontal patients because bacterial plaque is an etiologic factor in the development of periodontitis and the presence of orthodontic appliances facilitates plaque growth and maturation
Summary
We compared the differences in peptide mass fingerprints of orthodontic patients with and without periodontitis by MALDI-TOF MS using a magnetic bead-based peptidome analysis of saliva samples. In this way, we aimed to identify and explore a panel of differentially-expressed specific peptides. Patients who have chronic periodontal problems want to improve their malocclusions to obtain a perfect facial and tooth profile with orthodontic treatment, and their periodontal condition. The difference in osteogenesis between periodontitis and non-periodontitis patients [3] may lead to differences in Bacterial plaque is an etiologic factor in periodontitis [5]. Periodontal condition is usually determined visually by clinical periodontal and X-ray examinations, which are subject to considerable measurement error due to clinical experience, and are often poorly tolerated by patients
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