Magnet Integrated Fabric Phase Sorptive Extraction for the Extraction of Resin Monomers from Human Urine Prior to HPLC Analysis

Abstract

In this work, a method for the simultaneous determination of four resin monomers: Bisphenol A, bisphenol A methacrylate glycidate, triethyleneglycol-dimethacrylate, and urethane dimethacrylate, from human urine using magnet integrated fabric phase sorptive extraction (MI-FPSE), followed by high performance liquid chromatography (HPLC) diode array detection (HPLC-DAD), is presented. MI-FPSE is a novel configuration of FPSE that incorporates the stirring and extraction mechanism into one device, resulting in an improved extraction kinetic factor. FPSE is a green sample preparation technique that uses a flexible surface, such as cellulose, coated with a polymeric material using sol–gel technology. Poly(tetrahydrofuran) (PTHF) material was selected, due to its higher efficiency in terms of recovery rate among the studied MI-FPSE membranes. Optimization of the extraction process was performed based on several extraction and elution parameters. The method was validated for its linearity, selectivity, accuracy, precision, and stability of the samples. For the four compounds, the LOD and LOQ were 0.170 ng/μL and 0.050 ng/μL, respectively. The relative standard deviation of the method was less than 9.8% and 11.9%, for the within-day and between-day precision, respectively. The relative recoveries were between 85.6 and 105.2% in all cases, showing a good accuracy. The effectiveness of the proposed method was confirmed through successful application to the bioanalysis of real urine samples.