Magnesium Supplementation Combined With Hypoxic Training In Improving Hepatic Lipid Metabolism In Obese Mice

Abstract

PURPOSE: To explore the effect of hypoxic training on magnesium level, investigate the mechanism of magnesium supplementation combined with hypoxia training on hepatic lipid metabolism in obese mice. METHODS: Forty high-fat diet induced C57BL/6J mice were assigned into four groups (n=10 each): Diet-induced obesity (DIO); hypoxia training (HT), living and training in normobaric hypoxic conditions; magnesium supplementation (Mg), the 100mg/kg/d dose of magnesium chloride added to drinking water; hypoxia training+magnesium supplementation (HT+Mg). And set up a chow diet control group (CON). The intervention lasted for 4 weeks. All comparisons were made using two-way ANOVA analysis. RESULTS: 1) Serum magnesium in HT group (63.17±0.86 ppm vs 71.85±3.06 ppm) was decreased; femur magnesium in Mg group (2287.71±88.48 ppm vs 2106.89±41.68 ppm) were significant increased after 4 weeks intervention. And the femur magnesium was significantly negatively correlated with body weight (p<0.01, r=-0.645). 2) Compared with DIO group (1.80±0.32 g),liver weight of HT group(1.32±0.17 g),HT+Mg group (1.38±0.23 g) were significantly decreased; 3) Compared with DIO group (19.10±1.82 ng/ml,8.58±0.41 U/L),serum ATGL level in Mg group (25.43±1.71 ng/ml), HT+Mg group (31.56±2.46 ng/ml) and serum CPT-1 level in HT+Mg group (17.46±1.56 U/L vs) were significantly increased. 4) Compared with DIO group(1.01±0.19),liver PPARα mRNA expression in HT group (1.30±0.28), HT+Mg group (2.32±0.75) were significantly increased; liver ATGL (3.68±2.20 vs 1.04±0.31), LPL (7.72±3.14 vs 1.02±0.21), CPT-1 (6.85±3.89 vs 1.03±0.30) mRNA expression in HT+Mg group were significantly higher than DIO group. Moreover, the PPARα, CPT-1 mRNA expression level was significantly higher than HT group (1.43±0.46). CONCLUSIONS: 1) Hypoxic training can significantly reduce femur magnesium,increase the loss of magnesium while effectively losing weight. 2) 4 weeks of hypoxic training combined with magnesium supplementation intervention can effectively reduce the liver weight and hepatic free fatty acid in obese mice; 3) Hypoxic training combined with magnesium supplementation may promote liver lipolysis and fatty acid beta oxidation by activating liver PPARα and its downstream factors, improve liver lipid metabolism in obese mice.