Abstract
The aim of this study was to investigate the effect of magnesium sulfate (MgSO4) on experimental unilateral varicocele-induced in rats. Sixty male Wistar rats were randomly divided into six experimental groups (n = 10). The Control group had no received any medications and surgery. The Sham group had no received any medication, abdominal cavity was opened without varicocele-induced. Varicocele group: abdominal cavity was opened, varicocele done without any medication treatment. In group 4 abdominal cavity was opened, varicocele-induced then animals orally treated with MgSO4 (25 mg/kg) for 42 days. The groups 5 and 6 were similar to group 4, except animals received 50 and 100 mg/kg of MgSO4, respectively. At the end of days 21 and 42, the abdomen was opened, the left testis extracted for histopathological studies. Also, from the cauda epididymis semen samples were collected to determine malondialdehyde, superoxide dismutase and glutathione peroxidase values. According to the results, there was a significant difference in testis damage grade in Varicocele group compared to the Control group (P < 0.05). The MgSO4 significantly improved testis damage grade on day 42 (P < 0.05). The MgSO4 treatment, dose dependently improved seminiferous tubules with many spermatocytes in the seminiferous tubules in experimental unilateral varicocele established rats after 21 and 42 days (P < 0.05). Also, administration of the MgSO4 via a dose dependent manner significantly normalized malondialdehyde, superoxide dismutase and glutathione peroxidase values to the physiologic level in varicocele-induced rats after 21 and 42 days (P < 0.05). These results suggest administration of the MgSO4 protects testis against unilateral varicocele in rat.
Highlights
One of the most important disorders in the male reproduction system is varicoceles
To the best of our knowledge, there are limited studies describing the role of MgSO4 on spermatozoa characteristics in experimental varicocele in rat
This study was conducted for the first time to investigate the effect of MgSO4 on testis histopathology and semen MDA, superoxide dismutase (SOD) and glutathione peroxidase (GPx) in experimental unilateral varicocele-induced rat
Summary
One of the most important disorders in the male reproduction system is varicoceles It happens because of pathological dilations of the venous pampiniform plexus of the spermatic cord and frequently appears in the left side because left testicular vein runs vertically and inserts into the left renal vein (Naughton et al 2001; Sofikitis et al 2014). A relation exists between varicocele and semen oxidation where reactive oxygen species (ROS) levels increased and antioxidant capacity decreased in the semen of animal with varicocele (Hendin et al 1999; Hsieh et al, 2006). These changes lead to abnormal sperm function and the infertility (Masson and Brannigan, 2014). The low levels of ROS are critical for normal fertilization, capacitation, hyperactivation and motility (Agarwal et al, 2009)
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