Magnesium deficiency stress in rice can be alleviated by partial nitrate nutrition supply

Abstract

The problem of nitrogen (N) excess and magnesium (Mg) deficiency in farmland is becoming more common, severe, and widespread in southern China. Magnesium is known to be an essential nutrient for higher plants; however, the physiological responses of field crops to Mg deficiency, particularly to its interaction with N forms, remain largely unknown. In this study, a hydroponic experiment was conducted using three Mg levels (0.01, 1.00, and 5.00 mM) and three nitrate/ammonium ratios (NO3−/NH4+ of 0/100, 25/75, and 50/50) under greenhouse conditions. The results show that Mg deficiency (0.01 mM) could result in yellow leaves, dwarf plants, and fewer tillers during rice growth. Furthermore, Mg deficiency induced a major reduction in root morphology and activity, photosynthetic properties, and nutrient accumulation, while it resulted in a clear increase in malondialdehyde, superoxide dismutase, peroxidase, and catalase activities in rice. However, under Mg-deficiency stress, the supply of partial NO3− led to a significant drop in these antioxidant enzyme activities. Moreover, partial NO3− supply significantly improved the net photosynthetic rate, transpiration rate, stomatal conductance, and intercellular CO2 concentrations under Mg-deficiency conditions. In particular, the supply of partial NO3− dramatically promoted the growth of the root system, boosted the occurrence of lateral roots, and enhanced root vitality under Mg-deficiency stress. Additionally, the supply of partial NO3− led to significant increases in dry weight and N and Mg contents under Mg deficiency. The results of this study suggest that the symptoms of Mg-deficiency stress in rice can be alleviated by partial NO3− supply.