Abstract
Pollen transcript profiling of mutants defective in MADS-domain MIKC* protein complexes suggests they control a transcriptional network directing cellular differentiation during pollen maturation.
Highlights
Differentiation processes are responsible for the diversity and functional specialization of the cell types that compose an organism
AtMIKC* mutant characterization We previously described plants obtained from the Salk collection with a transferred DNA (T-DNA) insertion in the AGL65, AGL66, AGL94 and AGL104 genes, as well as three double mutant combinations of these insertion mutants [28]: agl65/ 66, agl65/104, and agl66/104
83.5% of the upregulated genes peak during the three immature stages. (b) The AtMIKC* complexes contribute quite significantly to the transcriptional changes that occur during pollen maturation. (c) We ranked all genes that were consistently called present in WT pollen according to their expression level in mature WT pollen, in descending order
Summary
Differentiation processes are responsible for the diversity and functional specialization of the cell types that compose an organism The outcome of these processes can be studied at molecular, physiologic, and biochemical levels by comparing different cell types, but the complexity and dynamics of the regulatory processes that specify the differentiation are largely unexplored. Pollen development proceeds along a stereotypical and unbranched pathway of differentiation, with well defined developmental stages, and is relatively synchronized within the anther, enabling the collection of homogenous cell populations at distinct stages of differentiation [11] These features make the male gametophyte an attractive model for detailed analysis of the cell differentiation process in plants
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