Madi‐Ryuk and its active compound tannic acid suppress allergic inflammatory reactions in activated human mast cell HMC‐1

Abstract

Madi-Ryuk (MDR) has been used for the treatment of arthritis as a health functional food and it alleviated clinical symptoms of patient with allergic disease. However, its scientific evidences have not been elucidated yet. Here we determined anti-allergic inflammatory effect of MDR and its constituent tannic acid (TA) in activated human mast cell line HMC-1 cells. In activated HMC-1 cells, MDR and TA reduced thymic stromal lymphopoietin (TSLP) protein release and mRNA expression without affecting cytotoxicity. MDR and TA blocked activities of caspase-1, nuclear factor-κB, mitogen-activated protein kinases, which are intracellular signaling pathways associated with increased TSLP levels in activated HMC-1 cells. Levels of inflammatory cytokines were reduced by MDR and TA on activated HMC-1 cells. Furthermore, MDR and TA reduced the nitric oxide production on activated macrophages. In conclusion, we clarified that MDR has a new anti-allergic inflammatory effect through reducing TSLP. Our results suggest that MDR may be useful in the treatment of allergic inflammatory diseases. Practical applications A health functional food, MDR has been clinically used for the treatment of arthritis. In addition, MDR has an anti-allergic inflammatory effect through the blocking the inflammatory reaction signaling pathways. Therefore, our results indicate that MDR may be useful in the prevention and treatment of allergic inflammatory diseases.