Abstract
The polypeptide of Sipunculs nudus was prepared by papain. The optimum papain enzymolysis process to Sipunculs nudus was confirmed by means of single factor analysis and orthogonal test, it showed as below: 300U/g of enzyme addition amount, 50°C of temperature, 60min of enzymolysis time and pH7.0. The clearance rate of hydroxy radical is 95.42% according to the optimum process on Sipunculs nudus enzymolysis. The HPLC chromatogram showed that the prepared component of antioxidative active polypeptide according to such condition is single and its molecular weight is 5868. The results of experiment shows that Sipunculs nudus polypeptide have high inoxidizability and high removing free radicals ability.
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