Macroscopic Modeling of Intracellular Trehalose Concentration in Saccharomyces cerevisiae Fed-batch Cultures

Abstract

This paper describes the extension of a baker's yeast growth model to account for the intracellular trehalose storage and mobilization. Trehalose is a reserve carbohydrate that is accumulated and converted back to intracellular glucose when the yeast cells face certain stresses. This is modeled by a new macroscopic reaction, which is coupled to an existing macroscopic reaction scheme describing the coordinated uptake of glucose and ammonium by the yeast cells. The dynamics of the trehalose concentration is described by a delay differential equation as the available experimental data used to fit the model exhibits a time-delayed correlation between trehalose storage and glucose uptake as well as a time-delayed correlation between trehalose mobilization and ethanol respiration phases. The proposed extension contains 6 parameters of which 5 are estimated via nonlinear least squares identification. The proposed model predicts accurately the dynamics of trehalose storage and mobilization and can be used to optimize the intracellular trehalose accumulation in Saccharomyces cerevisiae, which is valuable for obtaining reinforced yeast cells, able to better withstand drying operations.