Macroscopic and microscopic features of pancreatic scaffold generated by SDS-based decellularization using multiple needle injections

Abstract

Background: Pancreatic tissue engineering requires a scaffold in addition to cells and signaling. An adequate scaffold no longer contains cells but retains its extracellular matrix. Biological scaffolds from organ or tissue decellularization are widely used because they can retain the extracellular matrix essential for cell proliferation and differentiation. Pancreatic scaffolds were developed for pancreatic tissue engineering through various decellularization techniques. Available decellularization techniques have advantages and disadvantages, and the development of new techniques is necessary. This study aims to characterize pancreatic scaffold generated by SDS-based decellularization using multiple needle injections. Method: The rat pancreas was isolated and injected with multiple SDS with graded concentration (0,1-1%) using a 1 cc syringe. Scaffold characterization was performed using hematoxylin-eosin (H&E) staining. Results: The results obtained were the loss of cells in the pancreatic scaffold with intact extracellular matrix. Macroscopic appearance after decellularization showed a translucent, white, and flabby pancreas. Microscopic image after decellularization showed pancreatic tissue with cell loss but visible extracellular matrix. Conclusion: This study can produce an SDS-based decellularized pancreatic scaffold using multiple needle injections with adequate macroscopic and microscopic characteristics.