Macropodid marsupial luteinizing hormone: validation of assay procedures and changes in concentrations in plasma during the oestrous cycle in the female tammar wallaby (Macropus eugenii).

Abstract

A heterologous double antibody radioimmunoassay employing a rabbit anti-ovine LH antiserum (GDN no. 15) has been developed for the assessment of concentrations of LH in macropodid marsupial pituitary extracts and plasma. In this radioimmunoassay system highly purified ovine, rat human and kangaroo LH preparations demonstrated apparently parallel dose-response curves, as did serial dilutions of crude pituitary extracts from a wide range of Austrlian marsupial species and serial dilutions of plasma from ovariectomized, oestrous and LH releasing hormone (LH-RH)-treated marsupials. The assay has been used to monitor changes in immunoreactive LH in the plasma of the female tammar wallaby. Basal concentrations of LH in non-oestrous female wallabies were in the range < 0.20--1.90 ng/ml with many animals having values at or near the limit of detection of the assay. Concentrations of LH were markedly increased following ovariectomy (1.7--7.0 ng/ml), on the day of oestrus (10.0-- > 50 ng/ml) and following administration of LH-RH (9.5-- > 25.0 ng/ml). Plasma from hypophysectomized animals had no detectable LH immunoactivity, A well-defined LH surge, lasting approximately 12 h, was associated with oestrus. Mating occurred approximately 8 h before, and ovulation approximately 24 h after, the maximal concentrations of LH in plasma.