Abstract
AbstractPurpose: To analyse the effect of a systemic inflammatory insult induced by lipopolysaccharide (LPS) administration on the cornea in mice.Methods: Male and female C57BL/6J mice were injected with a single intraperitoneal injection of LPS (5 mg/kg). Intact animals were used as control. At 21 days after LPS administration animals were sacrificed with sodium pentobarbital. Corneas were dissected as flat mounts or histological sections (16 μM) and Iba1 (microglia and macrophages) and CD45 (activated microglia and macrophages) immunodetected.Results: In LPS treated animals but not in intact ones, microglia/macrophages were found in all corneal layers while in corneal flat mounts Iba1 + Cd45+ cells were observed through the corneal surface with higher density in the periphery.Conclusions: Sepsis causes infiltration and activation of microglia/ macrophages in the murine cornea that lasts up to 21 days.
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