Abstract
The influence of refrigerated storage on the macronutrients, total aerobic bacterial counts (TAB), as well as the milk serum proteome in expressed breast milk from 0 to 72 h was studied. Protein content decreased significantly (p < 0.05), whereas reducing sugars and TAB increased significantly after 48 h (p < 0.05). Fat content did not change significantly (p ≥ 0.05). In the human milk serum proteome, 253 proteins were identified and 110 proteins could be quantified. Among the quantified proteins, 22 proteins changed significantly after 48 h, such as xanthine dehydrogenase/oxidase, fatty acid-binding protein, and α-enolase, which were mainly involved in catalytic activity. On the other hand, immune-related proteins, lactoferrin and α-lactalbumin did not change significantly after 48 h between 4.9 and 6.0 °C. The results will help guide recommendations for the safe storage of human breast milk.
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