Macromolecular Synthesis in Cells infected with Reovirus Type 2 and the Effect of Ara-C

Abstract

WE have shown that it is possible to demonstrate in RA cells (an established human amnion culture) and in HeLa cells infected with reovirus type 2 the synthesis of double stranded viral RNA as early as 4–5 h after infection1,2. Although we did not observe any inhibition of cellular RNA synthesis, it was possible to distinguish viral RNA by its relative resistance to treatment with ribonuclease. This communication reports the macromolecular synthesis of DNA and protein in RA cells undergoing a single cycle infection with reovirus.