Macromolecular synthesis in cells infected with frog virus 3: V. The absence of polyadenylic acid in the majority of frog virus 3-specific mRNA species

Abstract

Most of the virus-specific RNA molecules isolated from the cytoplasm of BHK-21 cells infected with frog virus 3 (FV 3) did not contain poly(A) tracts of sufficient length to permit the RNA to bind to oligo(dT) cellulose. The 14% of [ 3H]adenosine-labeled RNA molecules that did bind were 40–55% RNAse-resistant and sedimented at about 6 S. In contrast, 40–70% of the pulse-labeled RNA isolated from vaccinia virus-infected BHK cells was bound to oligo(dT) cellulose, and the bound RNA was representative of the whole spectrum of vaccinia virus RNA size classes.