Abstract
Murine thymus and spleen cells synthesize a 225,000 dalton macromolecule (MICG). This protein is insoluble in the cold in non-ionic detergents and migrates as a β-globulin on electrophoresis. MICG can be isolated by a two-step procedure consisting of cold precipitation of cellular lysates in NP-40, followed by gel chromatography in detergent solution. Thymocytes synthesized three times more MICG than splenocytes. Immunological analysis demonstrated that anti-MICG antiserum reacted with the cold insoluble protein. Trypsin digestion of radiolabeled, isolated MICG illustrated the incorporation of 14C-leucine into a number of peptides. MICG is a glycoprotein which accounts for 3.5% of the protein synthesized in thymus cells and is not secreted from thymocytes or spleen cells.
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