Abstract
Macromolecular Fragments of Canine Cardiac Myosin Obtained by Tryptic Digestion
Highlights
MethodsPreparation of Cardiac Myosin-Procedures previously described [14, 15] were used with minor modifications for the preparation of myosin from dog heart
The first sign of a further fragmentation of heavy meromyosin consists in a blunting of the trailing edge of the heavy meromyosin peak (Fig. 1, 10 minutes of digestion)
These were indistinguishable in their sedimentation behavior, from the characteristic boundaries of these components in the sedimentation diagrams of digested cardiac myosin and concluded that no macromolecular fragments were produced
Summary
Preparation of Cardiac Myosin-Procedures previously described [14, 15] were used with minor modifications for the preparation of myosin from dog heart. Minced cardiac muscle was extracted with 3 volumes of 0.3 M KCl-0.075 M KH2POI-. 0.075 M K?HPOd-0.5 mM ATP, pH 6.6. Extraction was stopped after 15 minutes by straining the mince through cheesecloth, United States Public Health Service, National Institutes of Health, No HE 01422, Bethesda, Maryland; American Heart Association, Inc., No 61 G 139, New York, New York, and Health Research Service Foundation.
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