Abstract
Beamline X25 at the NSLS is one of the five beamlines dedicated to macromolecular crystallography operated by the Brookhaven National Laboratory Macromolecular Crystallography Research Resource group. This mini-gap insertion-device beamline has seen constant upgrades for the last seven years in order to achieve mini-beam capability down to 20 µm × 20 µm. All major components beginning with the radiation source, and continuing along the beamline and its experimental hutch, have changed to produce a state-of-the-art facility for the scientific community.
Highlights
X25 is a facility beamline operated by the Macromolecular Crystallography Research Resource group (PXRR) and the Photon Sciences Directorate at the 2.8 GeV National Synchrotron Light Source (NSLS) at Brookhaven National Laboratory (BNL)
Despite having to control slightly different hardware from one PXRR beamline to the other, the GUI remains the same throughout, making it easy for users to migrate from one beamline to another
X25 has been productive in publications and Protein Data Bank (PDB) depositions (Fig. 5) over its existence and accommodates around 100 different groups a year with roughly 11000 crystals mounted per year
Summary
X25 is a facility beamline operated by the Macromolecular Crystallography Research Resource group (PXRR) and the Photon Sciences Directorate at the 2.8 GeV National Synchrotron Light Source (NSLS) at Brookhaven National Laboratory (BNL). Major upgrades have been ongoing in order to promote the use of a mini-beam to match the trend of smaller biological samples. This beamline is used in tandem with X29 (Shi et al, 2006), an undulator beamline, and together they accommodate most of the life science users who make up 40% of the users coming to this synchrotron light source
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