Abstract
Macrolide-resistant Mycoplasma pneumoniae (MRMP) is highly prevalent in Asia and is now being reported from Europe. Few data on MRMP are available in the United States. Using genotypic and phenotypic methods, we detected high-level MRMP in 13.2% of 91 M. pneumoniae–positive specimens from 6 US locations.
Highlights
Macrolide-resistant Mycoplasma pneumoniae (MRMP) is highly prevalent in Asia and is being reported from Europe
We found a 23S rRNA point mutation A2063G known to confer macrolide resistance in 10 (10.9%) of 91 specimens by direct real-time PCR with melting curve analysis
MRMP prevalence has been reported to range from 2% to 26% in European countries [7,8] and is 30% in Israel [9]
Summary
Macrolide-resistant Mycoplasma pneumoniae (MRMP) is highly prevalent in Asia and is being reported from Europe. Testing for 23S rRNA mutations conferring macrolide resistance was performed on original specimens by real-time PCR melt curve analysis [6] and confirmed by DNA sequencing at the Lurie Children’s Hospital (Chicago). Subculture and phenotypic antimicrobial susceptibility testing was performed by using a broth microdilution method approved by the Clinical and Laboratory Standards Institute at the University of Alabama at Birmingham. We found a 23S rRNA point mutation A2063G known to confer macrolide resistance in 10 (10.9%) of 91 specimens by direct real-time PCR with melting curve analysis.
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