Macroglia distribution in the developing and adult inferior colliculus

Abstract

Macroglia distribution in the developing and adult gerbil inferior colliculus (IC) was investigated using two oligodendrocytic [myelin-associated-glycoprotein (MAG) and oligodendrocyte-specific molecule (Rip)] and two astrocytic [glial fibrillary acidic protein (GFAP) and S100] markers and immunohistochemistry. There was a spatio-temporal pattern of myelin marker expression starting in the ventral area of the IC and continuing to the dorsal part of the nucleus. Myelination, as revealed by MAG and Rip markers, starts in the IC during the second postnatal week. The intensity of myelination increased between stages P15 and P21 and extended to the whole IC. The appearance of myelin proteins in the IC may suggest a possible axonal outgrowth inhibition by oligodendrocytes in this structure. A differential pattern of staining was obtained with S100 and GFAP antibodies. Astrocytes identified as S100 immunoreactive cells were observed in the IC by birth and the staining was localized to their cell body and processes. S100 positive cells were homogeneously distributed within the IC nucleus. S100 pattern of staining remained the same in stages P7, P15 and P21. In adult IC, S100 positive cell processes were in contact with neuronal cell bodies, other S100 positive cells and blood vessels. Quantitative analysis showed an increase in the density of positive cells during the first postnatal week and a decrease then after through to adulthood. Unlike S100, GFAP immunoreactivity showed a different pattern of staining. At birth GFAP positive astrocytes were observed along the collicular brain midline and around the IC nucleus delimiting its boundaries. The GFAP pattern of labelling remained the same during development and in the adult. This data suggests the presence of two astrocytes subtypes with different locations in the IC nucleus. The GFAP positive astrocytes were located along the edge of the nucleus, while the S100 positive ones displayed a homogeneous distribution across the nucleus.