Abstract
Potentiometric detection of lipophilic drugs such as clenbuterol, ambroxol and bromhexine in standard-bore cyano and narrow-bore hybrid, reversed-phase HPLC systems was developed to enable specific determination of these mucolytic agents in some marketed oral-formulations. The macrocycle and podant-type, neutral ionophores, respectively: hexakis(2,3,6-tri-O-octyl)-α-cyclodextrin (CDX) andN,N,N′,N′-tetracyclohexyl-oxybis(o-phenyleneoxy)diacetamide (TOPA), were used to construct PVC-based, liquid membrane electrodes. In acidic aqueous-acetonitrile, mobile phases both ionophores improved the sensitivity of these electrodes for mucolytic agents when compared to single-wavelength UV detection—as well as with the ionophore-free based electrode. In the case of bromhexine, indicating highest lipophilicity (logP6.40), detection limits of 2.6×1010 and 1.4×1010M (injected concentration) were achieved with a signal-to-noise (S/N) ratio of 3 with the TOPA-modified, liquid membrane electrode in the various RP-HPLC systems. The results of procedures validated for simultaneous potentiometric determination of clenbuterol and ambroxol in tablets marketed in different countries are also presented.
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