Macro-scale acoustophoretic separation of lipid particles from red blood cells

Abstract

Autologous blood salvage is frequently used in cardiac surgery. However, shed mediastinal blood contains lipid particles ranging in size from 10 to 60 μm. Lipid emboli flow subsequently lodge in the brain capillaries resulting in strokes, leading to neurocognitive dysfunction and death. A novel acoustophoretic filtration system has been developed to separate the lipids from the red blood cells (RBCs). The system works at the macro-scale, supporting flow rates in excess of 2 L/h. The system is designed such that the acoustic radiation force is able to overcome the combined effects of fluid drag and buoyancy forces. Both RBCs and lipid particles are therefore trapped in the ultrasonic standing wave. Due to the opposite contrast factors of lipids and RBCs, the two components separate at opposite nodes within the standing wave, with lipids concentrating at pressure anti-nodes and RBCs at pressure nodes. Subsequent gravitational separation is used to separate the lipids and RBCs. Preliminary results were obtained with a suspension of 10x diluted bovine blood mixed with a 0.75% safflower oil emulsion. Measurements indicate a 15 fold increase in hematocrit of the captured RBCs when compared to the original sample solution, and an excellent separation of the oil droplets.