Macro- et microdosage de traces de cobalt. VI. Dosage du cobalt dans le sang

Abstract

A quantitative method for the analysis of cobalt in 50 to 100 g of normal whole blood, without anticoagulants, by spectrophotometry using nitroso-R salt, is discussed. By the use of radiocobalt 60 as a tracer, the loss can be estimated. With a final volume of 0.8 ml of solution, the spectrophotometric measurement is carried out on 0.4 ml contained in a microcell placed on a suitable support, as already described in a previous paper. The quantity of cobalt contained in a sample of normal blood is found to be 0.33 ppb with a relative error of ± 10%. The time necessary for one such assay is about 22 hours, of which 12 are without any special control; that for a series of 10 analyses is not sensibly different.