Abstract
This study presents a pragmatic and easily scalable maceration-mediated liquid-liquid extraction (MMLLE) and reverse-phase high-performance liquid chromatography (RP-HPLC)-based determination of Silybins from plant material (Curcuma longa L.). The processing of calibration standards revealed that the RP-HPLC method was linear over a concentration range of 1-100μg/mL with regression coefficient (R2)>0.9950, limit of detection 0.02μg/mL and limit of quantification <0.07μg/mL. The optimum chromatographic conditions resolved Silybin A, Silybin B, Isosilybin A and Isosilybin B within 5min of analysis time. The reproducible recovery rates of spiked flavonolignans (96.24-115.40%) from quality controls established the effectiveness of MMLLE procedure prior to HPLC determination. The real-time analysis revealed the presence of silybins in C. longa roots. The results further endorse that MMLLE prior to chromatographic determination may provide a more pragmatic analytical solution for the analysis/isolation of silybins.
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