MACE-Sequencing-Based Total RNA Expression Profiling of Breast Cancer: The miR-4510 Tumor Suppressor Confirmed as a Novel Biomarker

Abstract

Abstract: In the present study, we aimed to show the differential expression profiles of protein-coding RNAs and short non-coding (snc) RNAs in formalin-fixed, paraffin-embedded (FFPE) tissues of the breast cancer (BC) and normal adjacent tissue (NAT). We also aimed to identify miR-4510 expression level as a novel biomarker for the BC diagnosis and identify IGF1R, KLK4, LENG8, and CD44 as novel target genes for miR-4510. Methods: MACE-sequencing, TrueQuant, and qRT-PCR methods were applied for profiling the differential expression of mRNA transcripts, sncRNAs, and miR-4510, respectively. Depending on 11 target prediction tools, miR-4510 target predicted genes were identified. Results: Among 26,795 genes, 6998 chosen genes, with a p-value T) within the seed sequence was hypothesized to be affected by the mRNA;miR interaction and led to alterations in the breast cellular processes at various levels of gene regulation. Conclusion: The miR-4510 can be used as a novel marker for diagnosing the BC. IGF1R, KLK4, LENG8, and CD44 may be the novel targets for the miR-4510. Poly-miRTSs within the miR-4510 mature sequence may be the major cause for the BC development. Funding Statement: This study was supported by Sevan Majed as he is a Ph.D. student. Declaration of Interests: The author announces no conflict of interest. Mr. Sevan is a teacher of Salahaddin University-Erbil, a subsidiary of ministry of higher education in Kurdistan region government (KRG). Ethics Approval Statement: Prior to the study, all procedures while conducting the study were approved by the Human Research Ethics Committee (HREC) at the Science College at Salahuddin University-Erbil (Reference No. 4d/132). Publication permission was obtained by signing the confirmed consent of all research patients. All methods were also carried out in accordance with the Helsinki Declaration of 1964 during the study, and there was written informed consent and permission for publication for all research patients.