MA18.11 SNAI2 Is the Most Highly Differential Expressed Genes Between the Adenomatous and Squamous Components of Lung Adenosquamous Cell Carcinoma

Abstract

Lung adenosquamous cell carcinoma is a hybrid tumor with adenomatous and squamous components in one tumor, it has the worst prognosis among the non small cell lung cancer. Our previous work has prooved that lung adenosquamous cell carcinoma has a similar EGFR mutation rate as lung adenocarcinoma which is 51.79% in 56 patients. Further squencing results showed that the majority of adnematous and squamous components had identical mutation type (34/37). EGFR mutated adenosquamous cell carcinoma patients are prone to be young, female and non-smokers, which are similar with the clinical charateristics of lung adenocarcinoma. Besides the corcodant mutation profile between the two different components of lung adenosquamous cell carcinoma, if there are any differentail expressed genes remained mysterious. our study will addressed this question. NanoString nCounter technology was employed in our study. it has been increasingly used for mRNA or miRNA differential expression studies because of its advantages of feasibility in formalin fixed paraffin embedded samples. We compared the differential expressed gene profiles between the paired matched adenomatous and squamous components in 24 adenosquamous cancer tissues. SNAI2 is found to be the most differential expressed genes between the two components in lung adenosquamous cell carcinoma. It is higher expressed in squamous component compared with the adenomatous component. SNAI2 is a member of epithelial to mesenchymal transition signalling pathway. Other research results showed that SNAI2 played a pivotal role in controlling the epithelial and mesenchymal transdifferentiation, stem cell property of cancer cell, and the metastasis ability. Our preliminary results showed that SNAI2 which is a member of EMT signalling pathway is highly expressed in the squamous component compared with the adenomatous component of lung ASC. It may contribute to the phenotype transdifferentiated and invasion of lung ASC.