M1709 Analysis of Proliferative/Apoptotic Ratio, Mitotic Index and Apoptotic Index in Histologically Intact Children Colonic Biopsy Samples and Colorectal Adenoma-Dysplasia-Carcinoma Sequence in Adults

Abstract

The number of EGFR and HGFR positive subepithelial cells was found to be significantly elevated in severe (21.6±2.1%/21.3±1.9%), moderate (14.3±1.7%/14.6±1.6%) and mild (7.2±1.6%/7.4±1.3%) inflammation compared to healthy colon mucosa (2.6±1.4%/ 2.4±1.03%) (p<0.005). IGFR1 immunoreactive cells were only found in a trace number in the subepithelial layer in all cases. Low number of CDX2 and CK positive subepithelial cells were also found in ILFs, and their increase in number was in correlation (r=0.8) with the severity of inflammation. RT-PCR validated the immunohistochemical results. CONCLUSION: EGFR/HGFR positive subepithelial cells showing numerical correlation with the activity of inflammation in ulcerative colitis may indicate their involvement in regenerative mucosal processes. The presence of CDX2 or CK positive subepithelial cells suggest that the mesenchymal-epithelial transition may be located to ILFs.