Abstract
Methods Immunodominant regions of 3 Mycobacterium tuberculosis specific cell–wall proteins that are highly immunogenic in HIVTB+ and HIV+TB+ patients have been mapped. Antibodies to these regions are absent in subjects with latent TB, BCG vaccination, other pulmonary diseases. To examine the feasibility of devising a peptide based rapid POC test, selected peptides were conjugated to a carrier protein using standard EDC/NHS coupling chemistry, purified by size exclusion; the peptide conjugation confirmed using MALDI MSMS before adsorption onto colloidal gold and striping onto nitrocellulose. The assay parameters were adjusted to determine positive/negative status within 15 minutes via visual or instrumented assessment.
Highlights
test that can be implemented in peripheral health care settings to replace
epitopes of highly antigenic M. tuberculosis specific proteins can be the basis of antibody detection based rapid point-of care (POC) TB test
Test optimization to improve accuracy to meet WHO specifications for a POC test to replace microscopy
Summary
Jesus M Gonzalez[1,2], Bryan Francis[1], Sherri T Burda[3], R Sam Niedbala[1,2], Suman Laal1,3,4*. From 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014) Chennai, India. From 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014) Chennai, India. 30 January - 1 February 2014
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