Abstract
A simple and inexpensive microtitre plate test (the mixed reverse (solid-phase) passive antiglobulin haemadherence test, or M RSPAH) has been developed for the measurement of antiglobulins (RFs) of different classes. Results obtained for IgM RF by this test have been compared with results of latex and Rose-Waaler (DAT) tests on rheumatoid arthritis (RA) sera. Levels of RFs of IgM, IgG and IgA classes have been measured by M RSPAH using rabbit IgG as antigen in RAs and normal people. 94% of RA sera tested were above the upper limit of normal for IgM and/or IgA RF. There was a considerable overlap between IgG RF levels in RAs and normals, although the means of the two groups were significantly different.
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