M intermediate accumulation analysis of bacteriorhodopsin reconstituted with three partial peptides

Abstract

Reconstituted bacteriorhodopsin containing helices C-G, A, and B has unique thermal structural change and photoreaction. Time evolution of photo reaction of bacteriorhodopsin reconstituted with three partial peptides in glycerol mixed purple membrane at around 196 K under irradiation by red light was analyzed by a proposed model. The absorption change of 560 nm and 405 nm with irradiation times was analyzed for the purpose of determining reaction rates of photo reaction of reconstituted bacteriorhodopsin and its product M intermediate. In this study it is shown that reaction rates of conversion from bacteriorhodopsin reconstituted with three partial peptides to the M intermediate can be explained by a set of linear differential equations. Although the rate of accumulation of M intermediate is as forty times smaller than that of the native bacteriorhodopsin, the accumulation was achieved, in spite of absence of peptide bond between peptides.