M-carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (Bubalus bubalis) embryos.

Abstract

Incomplete or aberrant reprogramming of nuclear genome is one of the major problems in somatic cell nuclear transfer. In this study, we studied the effect of histone deacetylase inhibitor m-carboxycinnamic acid bishydroxamide (CBHA) on in vitro development of buffalo embryos produced by Hand-made cloning. Cloned embryos were treated with CBHA (0, 5, 10, 20 or 50μM) for 10hr from the start of reconstruction till activation. At 10μM, but not at other concentrations examined, CBHA increased (p<.05) the blastocyst rate (63.77±3.97% vs 48.63±3.55%) and reduced (p<.05) the apoptotic index of the cloned blastocysts (8.91±1.94 vs 4.36±1.08) compared to untreated controls, to levels similar to those in IVF blastocysts (4.78±0.74). CBHA treatment, at all the concentrations examined, increased (p<.05) the global level of H3K9ac in cloned blastocysts than in untreated controls to that observed in IVF blastocysts. Treatment with CBHA (10μM) decreased (p<.05) the global level of H3K27me3 in cloned blastocysts than in untreated controls but it was still higher (p<.05) than in IVF blastocysts. CBHA (10μM) treatment increased (p<.05) the relative expression level of pluripotency-related genes OCT-4 and NANOG, and anti-apoptotic gene BCL-XL, and decreased (p<.05) that of pro-apoptotic gene BAX than in untreated controls but did not affect the relative expression level of apoptosis-related genes p53 and CASPASE3 and epigenetics-related genes DNMT1, DNMT3a and HDAC1. These results suggest that treatment of cloned embryos with 10μM CBHA improves the blastocyst rate, reduces the level of apoptosis and alters the epigenetic status and gene expression pattern.