Lysyl Oxidase-Like Protein-2 Silencing Suppresses the Invasion and Proliferation of Esophageal Cancer Cells

Abstract

This study explores the effect of silencing lysyl oxidase-like protein-2 (LOXL2) gene on TE-1 cells. TE-1 cells were transfected by LOXL2-siRNA. E-cadherin, LOXL2, and Snail were detected using Western blot and Real-time PCR. Transwell invasion and migration assay was performed. Flow cytometry detected apoptosis. Cell growth was analyzed with CCK-8 and colony formation. After48 h of transfection, compared with control groups, LOXL2 mRNA in the LOXL2-siRNA group (0.40±0.01) lowered significantly (P < 0.05). Consistently, LOXL2 protein in LOXL2-siRNA group was (0.48± 0.02), significantly lower than that in blank control (1.04± 0.03) and negative control (1.02± 0.02) (P < 0.05). After 72 h of cell culture, the absorbance of LOXL2-siRNA group was (0.43±0.04), which reduced significantly than blank control (0.81±0.05) and negative control (0.84±0.06) (P < 0.05). Similarly, cell clone number after LOXL2-siRNA transfection (72.3±4.2)increased significantly than the negative control (178.8±4.6) and blank control (167.3±3.5) (P < 0.05). However, LOXL2 silencing did not significantly affect cell apoptosis. Furthermore, LOXL2 silencing inhibited Snail while increased E-cadherin (P < 0.05). Conclusively, LOXL2 silencing may suppress the invasion and proliferation of esophageal cancer cells via down-regulating Snail, and up-regulating E-cadherin to inhibit EMT in esophageal cancer cells.