Lysozyme activity in animal extracts after sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Abstract

1. 1. Lysozyme activity was detected after electrophoresis in sodium dodecyl sulfate-polyacrylamide gels containing 0.2% (W/V) autoclaved Micrococcus lysodeikticus cells as substrate. 2. 2. Lysozyme activity appeared as clear lysis zones after incubation of opaque gels at 37°C in buffered Triton X-100. 3. 3. As low as 0.1 pg of purified hen egg white lysozyme could be detected after 16 hr incubation at pH 6.5. 4. 4. Bands with lytic activity from kidney and pancreas acetone powders, bird's egg whites and vitelline membranes, animal sera and human saliva corresponded to c-type ( M r 14,500), g-type ( M r 20,500) or both lysozymes as far as molecular weight is concerned. 5. 5. Some extracts, like porcine kidney, exhibited more than two bands. 6. 6. Bands with lytic activity migrating at the level of g-type lysozymes were detected in some kidney and pancreas extracts.