Abstract
Background: Low-energy/low-dose ionizing radiation is increasingly being used in medical diagnosis, yet the effects of low-energy/low-dose medical diagnostic X-rays on biological systems remains a mystery. Thus, the aim of this present study is to determine the characteristics of lysosomes of cancerous and normal cells in response to low-energy/low-dose medical diagnostic X-rays.
 Methods and materials: Three cell lines composed of peripheral blood mononuclear cells (PBMCs), adriamycin-sensitive erythroleukemia cells (K562), and adriamycin-resistant erythroleukemia cells (K562/adr), were all exposed to low-energy/low-dose medical diagnostic X-rays that operated at 50, 70, and 100 kV, and 100 mAs for obtaining radiation doses at 0.03, 0.07, and 0.10 mGy, respectively. Accumulation of acridine orange in the lysosomes of each cell line was determined using flow cytometry completed at 4 and 6 hours-post irradiation.
 Results: The data showed an increase in fluorescence intensity of acridine orange in three irradiated cells line at 4 hours post-irradiation, but this intensity did not change at 6 hours post-irradiation when compared to that of corresponding non-irradiated cells.
 Conclusion: This finding suggests that changes in lysosomes might be a response to low-energy/low-dose medical diagnostic X-rays.
 Bangladesh Journal of Medical Science Vol.18(4) 2019 p.830-834
Highlights
It is well known that high doses of ionizing radiation can induce harmful health effects
There are several studies that have indicated that the effects of low dose and high dose ionizing radiation is dissimilar with regard to biological responses
Regardless of this evidence, the health risks of exposure to low dose ionizing radiation are still being examined, as low dose ionizing radiation is increasingly being used in medical diagnosis[1]
Summary
It is well known that high doses of ionizing radiation can induce harmful health effects. There are several studies that have indicated that the effects of low dose and high dose ionizing radiation is dissimilar with regard to biological responses. Regardless of this evidence, the health risks of exposure to low dose ionizing radiation are still being examined, as low dose ionizing radiation is increasingly being used in medical diagnosis[1]. Lysosomes are acidic intracellular organelles that contain several enzymes that can break down many kinds of intracellular molecules such as DNA, lipids, and proteins It acts as a waste elimination system for the cell by digesting unwanted molecules from extracellular and intracellular compartments in the cytoplasm.
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