Abstract
Pigmentary disorders generally indicate that an increased amount of melanin, leading to darker color of skin, called hypermelanosis. Thus, several methods were studied for finding therapy of hypermelanosis until now in worldwide. In this study, the hypermelanosis reduction has been observed with daily lysosome-related organelles (LRO) extract from eggwhite. The scrub was used for enzyme extraction so as to maintain the stability of the extracted enzyme. The LRO extracts were divided and into two groups: free enzyme and fresh enzyme. Free enzyme was extracted on the first day from the LRO and fresh enzyme was extracted by scrub from LRO daily. The melanin dissolved phosphate buffer (pH 7.0) was treated by two groups of enzymes for a period of 30 days. The fresh enzymes were found to be more effective than the free enzymes. The activity of free enzymes decreases with time and as a result it could not degrade melanin after 36 days. The peroxidase activity was also measured and it matched with the results of previous studies. Therefore, the daily fresh enzymes displayed remarkable ability in hypermelanosis reduction than with free enzyme that lost its activity after some time. The present work can be potentially used for dermal disease in medical industry and also in cosmetic products.
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