LYSOSOMAL ENZYMES IN BRUISED TISSUE.

Abstract

SummaryLysosomal DNase, RNase, β-glucuronidase and β-galactosidase activities in experimentally inflicted poultry breast bruises increased by the second day post contusion, reached a maximum between the third and fifth day and then declined toward normal as the bruises showed gross morphological healing. The magnitude of enzymatic response as a result of bruising was not the same for each enzyme, but all enzymes showed the same trends in activity. Activities were elevated to a greater extent in the center of the bruise than at the edge. Normal muscle from bruised chickens had activities similar to tissues excised from unbruised chickens. The effects of increasing the severity of muscular injury were to increase the maximum activity response and, also, the time required to reach it. Free, bound and total DNase, RNase and β-glucuronidase activities were determined as a function of age of bruise. All forms of the enzymes were found to increase during the healing process.