Lysosomal enzyme activity of monocytes/macrophages following incubation with postprandial hyperlipemic serum and its significance for the development of atherosclerosis

Abstract

Lipid accumulation in macrophages is a prominent feature of the atherosclerotic lesion. Decreased lysosomal function of these cells might play an important role in the pathogenesis of the atherosclerotic foam cell. In this investigation six normal volunteers were fed a meal with a high fat content (68.9% energy, P/S ratio 0.13). The hyperlipidemic postprandial serum was incubated with monocyte derived macrophages. The enzyme activity of cathepsin B, acid cholesterylester-hydrolase and N-acetyl-beta-hydrolase decreased significantly in these cells. Thus, inadequate response in enzyme activity of lysosomal enzymes in case of fat overload might contribute to the development of the atherosclerotic foam cell.