Abstract
The contents of the central vacuole (cell sap) were collected from internodal cells of Nitella by a modification of the procedure described by Tazawa (1964). Most of the acid phosphatase and carboxypeptidase, which are marker enzymes for lysosomes, were detected in the cell sap. In contrast, most of the catalase and cytochrome c oxidase were detected in parts of the cell other than the cell sap; this indicates there is relatively little contamination of the separated cell sap by the cytoplasm. It is suggested that the central vacuole of a mature plant cell possesses some of the properties of a lysosome.
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