Abstract
BackgroundTo study the role of lysosomal decomposition and elimination of old bone matrix, as well as the mechanism of promoting chondrocyte growth and bone recovery through the perspective of TFEB-mediated lysosomal autophagy.MethodsRat models of acute knee injury were designed, and autophagy flow was detected by injection of autophagy inhibitors 3-methyladenine. Autophagy flow was detected by RFP-GFP-LC3 double fluorescence molecule. The expression of TFEB, DRAM, MAPLC3, and MITF were analyzed by Western blot, and the expression of genes NITF, Bcl2, and TYR in rat cartilage tissues were detected by RT-PCR.ResultsThe number of autophagosomes was increasing in the auto group compared with the inhibitor-auto group and normal group. There was a significant difference of LC3 levels in the auto group and inhibitor-auto group compared with the normal control. The expression of TFEB, DRAM, MAPLC3, and MITF proteins by Western blot analysis were significantly increased in the auto group and decreased in the inhibitor-auto group. The expression of NITF, Bcl2, and TYR by RT-PCR determination were higher in the auto group and inhibitor-auto group than the normal group.ConclusionsAutophagy can inhibit apoptosis, promote chondrocyte growth and bone regeneration, and restore knee joint injury of rats. The main mechanism is to promote the effect of TFEB-mediated lysosomal autophagy.
Highlights
Lysosomes are organelles that decompose proteins, nucleic acids, polysaccharides, and other biological macromolecules [1]
Morphological characteristics of articular cartilage in rats The number of autophagosomes was increasing in the auto group compared with the inhibitor-auto group and normal group, while the number of inhibitor-auto group declined after using auto-inhibitor agent (Fig. 1)
The results showed that there was a significant difference of LC3 levels in the auto group and inhibitor-auto
Summary
Lysosomes are organelles that decompose proteins, nucleic acids, polysaccharides, and other biological macromolecules [1]. They are membranous organelles in protozoa and multicellular animal cells, while bacteria do not have lysosomes [2]. Autophagy is a conservative evolutionary process in which cell proteins and organelles are engulfed by autophagosomes and eventually transferred to lysosomes for degradation [3]. This research intends to study the role of lysosomal decomposition and elimination of old bone matrix, as well as the mechanism of. To study the role of lysosomal decomposition and elimination of old bone matrix, as well as the mechanism of promoting chondrocyte growth and bone recovery through the perspective of TFEB-mediated lysosomal autophagy
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