Lysophosphatidic Acid Stimulates MCP-1 Secretion from C2C12 Myoblast

Tamotsu Tsukahara,Hisao Haniu

doi:10.5402/2012/983420

Tamotsu Tsukahara, Hisao Haniu

Open Access

https://doi.org/10.5402/2012/983420

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Journal: ISRN inflammation	Publication Date: Nov 25, 2012
Citations: 33	License type: CC BY 3.0

Affiliation: Ōtani University

Abstract

Chemokines are regulatory proteins that play an important role in muscle cell migration and proliferation. In this study, C2C12 cells treated with lysophosphatidic acid (LPA) showed an increase in endogenous monocyte chemotactic protein-1 (MCP-1) expression and secretion. LPA is a naturally occurring bioactive lysophospholipid with hormone- and growth-factor-like activities. LPA is produced by activated platelets, cytokine-stimulated leukocytes, and possibly by other cell types. However, the LPA analog cyclic phosphatidic acid (cPA) had no effect on the expression and secretion of MCP-1. LPA, although similar in structure to cPA, had potent inducing effects on MCP-1 expression in C2C12 cells. In this study, we showed that LPA enhanced MCP-1 mRNA expression and protein secretion in a dose-dependent manner. Taken together, these results suggest that LPA enhances MCP-1 secretion in C2C12 cells and thus may play an important role in cell proliferation.

Highlights

Chemokines are a large family of structurally related proteins that play an essential role in leukocyte migration and differentiation [1]
A major finding in this study is that lysophosphatidic acid (LPA) activation in C2C12 cells may enhance Monocyte chemotactic protein 1 (MCP-1) expression and secretion
To detect cytokines and chemokines secreted by C2C12 cells after exposure to LPA, we measured the levels of 16 cytokines and chemokines and compared them with those in vehicle control

Summary

Introduction

Chemokines are a large family of structurally related proteins that play an essential role in leukocyte migration and differentiation [1]. LPA, similar in structure to cPA, had potent inducing effects on Monocyte chemotactic protein 1 (MCP-1) expression in C2C12 cells. Our study is the first to report that LPA induces MCP-1 secretion and increased cell proliferation in vitro. A major finding in this study is that LPA activation in C2C12 cells may enhance MCP-1 expression and secretion.

Results

Conclusion