Abstract
It has been demonstrated in LPA3 knockout mice that LPA3, a G protein-coupled receptor for lysophosphatidic acid (LPA), is involved in uterine receptivity. LPA3 is almost exclusively detected in the luminal endometrial epithelium at preimplantation mouse uterus. To investigate LPA receptor-mediated signaling on uterine receptivity, we use human luminal endometrial epithelial ECC-1 cell line as an in vitro model system. Preliminary data indicate that among the five LPA receptors identified up to date, LPA1, LPA2, LPA3, and LPA5 have significant levels of expression in the ECC-1 cells. LPA can induce ECC-1 cell proliferation and cell morphological changes. However, LPA doesn't induce ECC-1 cell migration. In addition to these cellular effects, LPA induces the expression of several genes implicated in uterine receptivity, such as LIF, COX-2, IL-6 and IL-8. LPA also induces phosphorylation of Akt and Erk1/2 in time and dose-dependent manner. The LPA downstream signaling pathways regulating the cellular effects and gene expression as well as the specific LPA receptor(s) in mediating these effects are being investigated in ECC-1 cells. (poster)
Published Version
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