Abstract
A phage-like particle (phage i) found in filtrates of Rhizobium trifolii (strain RT 9) did not produce plaques but induced development of two different phages in a presumed defective lysogenic strain (RT 10). These induced phages (phages 9 and 10) differed in their host ranges and produced plaques on strains RT 9 and RT 10, respectively. Phages 9 and 10 were closely related serologically and were distinct from phage i. Although ultraviolet irradiation resulted in the lysis of RT 10, no free phage was detectable in such lysates. Phage i particles had morphological, chemical, and physical properties similar to those of functional Rhizobium phages but did not produce plaques on any of 12 R. trifolii strains tested. Development of phage i could be induced with ultraviolet irradiation or by treatment with mitomycin C.
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