Abstract
This chapter presents specific methods that are commonly used to induce bacterial lysogens. Bacterial lysogeny is defined in the results of the intensive analyses of one temperate phage system. The chapter considers indirect ultraviolet induction in a detailed review of the phenomenon. Models for the lysogenic induction mechanism suggested by these many observations are juxtaposed, and, finally, analogies with virus-transformed mammalian cell lines are indicated. The biochemical definition of lysogenic induction has demanded detailed understanding of two requisites for lysogeny: continuing repression of particular early phage-specific activities, and the integration of the phage genome with that of its host. These molecular mechanisms have such complexity that our comprehension remains rudimentary. The multiplicity of inducing agents has also tended to compound the difficulty of clarifying these processes at the molecular level.
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