Lysobacter hymeniacidonis sp. nov., isolated from a crude oil-contaminated marine sponge

Abstract

An aerobic, Gram-negative bacterium, strain 2–5T, was isolated from a crude oil-contaminated marine sponge collected near Dalian Bay, China, and subjected to a polyphasic taxonomic investigation. Cells of strain 2–5T were non-spore forming, non-motile, rods 0.2–0.3 µm wide and 1.1–1.2µm long. Strain 2–5T grew well on nutrient agar, TSA, R2A agar and LB agar. Colonies of strain 2–5T on LB agar were circular, smooth with entire margins, non-transparent and pale yellow after 3 d of incubation at 30°C. Growth of strain 2–5T occurred in LN medium with 0–6% NaCl; no growth occurred in the presence of 8.0% NaCl. Strain 2–5T grew at 15–42°C and at pH 6.0–8.0. Comparative 16S rRNA gene sequence analysis showed that strain 2–5T clustered with the species of the genus Lysobacter. Its closet neighbors were the type strains of Lysobacter concretionis KCTC 12205T (97% similarity), Lysobacter arseniciresistens ZS79T (96%), and Lysobacter defluii APB-9T (96%). The value for DNA-DNA relatedness between strain 2–5T and L. concretionis KCTC 12205T was 23%. Branched fatty acids iso-C16: 0, iso-C15: 0, iso-C11: 0 3-OH, iso-C17: 1ω9c and iso-C11: 0 were found to be predominant. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Strain 2–5T had a DNA G+C content of 63.8 mol%. On the basis of the phenotypic, chemotaxonomic, DNA-DNA hybridization and phylogenetic data, strain 2–5T represents a novel species of the genus Lysobacter, for which the name Lysobacter hymeniacidonis sp. nov. is proposed. The type strain is 2–5T (=CGMCC 1.12190T = JCM 18137T).