Abstract
Abstract Immunologic injury to rabbit platelets provides a model system for the study of tissue damage mediated by unrelated immune complexes, complement and divalent cations (1, 2). Since numerous immunobiologic reactions require the Fc antibody fragment, it was of interest to determine whether the Fc portion is also essential for platelet injury. Recent evidence has indicated that the F(ab')2 fragments of guinea pig (3) and rabbit (4) immunoglobulins can activate the complement system via the alternate pathway. Immunoglobulins were obtained from the sera of rabbits hyperimmunized with human serum albumin (HSA)4 or ferritin. The γ-globulin fraction precipitated at 40% saturated ammonium sulfate served as intact antibody and was presumed to consist primarily of 7S immunoglobulins. Aliquots of these preparations were subjected to pepsin digestion according to the method of Nisonoff (5) followed by chromatography on Sephadex G-150 and concentration of the 5S eluate by pressure ultrafiltration. The antibody concentrations of the intact and pepsin-digested preparations were determined by quantitative precipitin analysis.
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