Abstract
The nontransformed, nontumorigenic CHEF/18 Chinese hamster embryo fibroblast line, as well as nontumorigenic CHEF/18 mutants that had become anchorage independent or acquired a reduced serum requirement for growth, and fully transformed, tumorigenic CHEF cell lines were analyzed for their sensitivity to killing in vitro by human natural killer (NK) cells. Nontumorigenic but transformed anchorage-independent and low-serum-requiring mutants remained insensitive to NK-mediated lysis like the parent CHEF/18 line. Only fully tumorigenic CHEF lines were found to be sensitive to NK-mediated lysis, although a few tumorigenic lines were resistant to NK lysis. These results indicate that NK sensitivity is not the result of any cellular changes associated with acquisition of an anchorage-independent or low-serum-requiring phenotype but is the result of some additional change(s) found only in fully tumorigenic CHEF cells. Our studies also show that, whatever the NK target structure is, it is evolutionarily conserved so that human NK cells are able to distinguish between Chinese hamster tumorigenic and nontumorigenic cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Proceedings of the National Academy of Sciences of the United States of America
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
